The genes encoding pyruvate decarboxylase ( pdc ) and alcohol dehydrogenase ii ( adh ii ) were amplified from total dna of zymomonas mobilis by pcr . the genes of adhb andpdc were inserted into expression vector pse380 and then transformed into e . coli dh5a . the recombinant strains were induced to express adh ii and pdc with iptg 本论文以zymomonasmobilisdna为模板， pcr扩增zymomonasmobilis中的乙醇脱氢酶基因( adhb )和丙酮酸脱羧酶基因( pdc ) ，分别构建表达质粒pse - adhb和pse - pdc并在大肠杆菌dh5中表达。
